Req #: 234295

Department: DEPARTMENT OF LABORATORY MEDICINE AND PATHOLOGY

Posting Date: 05/16/2024

Closing Info: Open Until Filled

Salary: $4,649 - $7,500 per month

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701_a11y.pdf )

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.

Research Scientist/Engineer 2 (E S UAW RSE) in this role will have a primary goal of supporting the research projects to quantitatively characterize the effects of cardiac protein defects on cardiomyocyte structure, sarcomere structure, cell and ventricular morphology, multiscale biomechanics, cell mechanobiology, and electrophysiology. These studies will help us to understand structural and functional consequences of pathogenic protein variants in cardiomyocytes, with the ultimate goal of discovering and understanding molecular and cellular bases of ventricular hypertrophy, contractile dysfunction, and heart failure.

DUTIES AND RESPONSIBILITIES Scientific Research Activities (80%)

• Under general supervision, design, perform, and troubleshoot experiments according to documented protocols and/or based on published literature.

• Assist in troubleshooting problems that arise in research protocols and report problems to the PI.

• Perform, assist, and train lab members in human induced pluripotent stem cell culture, cell line development and maintenance, directed differentiation into cardiomyocytes.

• Assist and train lab members in basic molecular biology assays, such as RNA/protein extraction, RT-qPCR, western blotting, plasmid design & transfections.

• Perform and train lab members in human stem cell gene editing techniques to generate new cell lines.

• Perform and train lab members in quantitative fluorescence microscopy.

• Develop novel stem cell-derived cardiomyocyte culture systems and environments for in-vitro biomechanics and mechanobiology studies.

• Perform and train lab members in cell line quality control, characterization, and phenotyping.

• Design, manage, and conduct experiments, analyze and interpret data, and communicate conclusions.

• Assist in writing and maintaining lab SOPs.

• Participate in writing manuscripts about new research findings, assay development, and continuing data collection and interpretation.

• Participate in presentations to peers and supervisor both at formal conferences, lab meetings, and informal meetings with colleagues.

• Maintain detailed lab records.

• Conduct literature reviews and background searches to assist in determining research methods and project planning.Other Duties (20%)

• Purchasing, inventorying reagents and supplies.

• General lab cleaning, maintenance, safety inspections.

• Lab member safety training.

• Operate ancillary equipment such as computers, printers, etc.

• Perform related duties as required.

• Attend and participate in lab meetings and seminars. MINIMUM REQUIREMENTS

• Bachelor's degree in Integrative Biology, Bioengineering, Mechanobiology or a related field AND two years of relevant experience, OR Equivalent combination of education/experience.

• Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. ADDITIONAL REQUIREMENTS

• Must have a deep understanding of stem cell biology, stem cell culture, differentiation, gene editing, cardiac biology, and cardiac pathology.

• Must have a strong knowledge of the related literature and current state-of-the-art methodologies/approaches for investigating cardiac mechanobiology and biomechanics in health and disease. DESIRED QUALIFICATIONS

• Ability obtain, analyze, and interpret next-gen sequencing data (RNAseq, ATACseq, ChIPseq, etc.) from stem cellderived cardiomyocytes.

• Able to generate engineered heart tissues (EHTs) and collecting/interpreting mechanics data from the EHTs.

• Ability to engineer novel cell culture techniques, environments, and/or apparatuses for biomechanical and mechanobiology investigations of human stem cell-derived cardiomyocytes. Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.